TRPC4 Polyclonal antibody

TRPC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21349-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Trp-related protein 4, TRP4, Trp related protein 4, hTrp-4, HTRP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21349-1-AP targets TRPC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC4 fusion protein Ag15698 Predict reactive species
Full Name transient receptor potential cation channel, subfamily C, member 4
Calculated Molecular Weight 977 aa, 112 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC104725
Gene Symbol TRPC4
Gene ID (NCBI) 7223
RRIDAB_10734426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBN4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC4 (Transient Receptor Potential Paradigm 4) is a non-selective calcium channel protein widely found in the nervous system and cardiovascular system. TRPC4 regulates intracellular calcium levels through the activation of signaling pathways mediated by Gq/11 and Gi/o-coupled receptors, and is involved in neurotransmission, neuronal excitability, and vascular endothelial cell function. In the nervous system, TRPC4 is associated with neuropsychiatric disorders such as depression and anxiety, and its inhibitors show antidepressant and anxiolytic potential. In the cardiovascular system, TRPC4 affects vascular permeability and diastolic function by regulating calcium ion inward flow and plays an important role in hypoxia-induced pulmonary hypertension. In addition, TRPC4 is involved in thermoregulation, sensing changes in internal temperature to regulate the drop in body temperature. aberrant expression of TRPC4 is associated with a variety of diseases, making it a potential therapeutic target!

Protocols

Product Specific Protocols
WB protocol for TRPC4 antibody 21349-1-APDownload protocol
IHC protocol for TRPC4 antibody 21349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Adv

Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.

Authors - Jingjing Duan
humanWB

Cancer Lett

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Authors - Da-Cen Lin
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