Tested Applications
| Positive WB detected in | Jurkat cells, mouse testis tissue, Raji cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 14 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
| CoIP | See 4 publications below |
Product Information
12205-1-AP targets TRIM27 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM27 fusion protein Ag2849 Predict reactive species |
| Full Name | tripartite motif-containing 27 |
| Calculated Molecular Weight | 513 aa, 58 kDa |
| Observed Molecular Weight | 58 kDa, 41 kDa |
| GenBank Accession Number | BC013580 |
| Gene Symbol | TRIM27 |
| Gene ID (NCBI) | 5987 |
| RRID | AB_2256660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14373 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM27 antibody 12205-1-AP | Download protocol |
| IHC protocol for TRIM27 antibody 12205-1-AP | Download protocol |
| IP protocol for TRIM27 antibody 12205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer. | ||
Mol Ther Nucleic Acids TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination. | ||
FASEB J TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells.
| ||
J Cell Mol Med NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop. | ||
J Leukoc Biol Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes. | ||
FEBS J TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Amy (Verified Customer) (03-18-2024) | Clean bands for U2OS cell lysates 25ug.
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FH Jason (Verified Customer) (08-17-2022) | Third time purchase. I am satisfied. This antibody works very well in human breast cancer cells using WB at dilution 1:1000, but many non-specific IF singling detected.
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FH Elisa (Verified Customer) (12-21-2021) | HAP1 were lysates with RIPA buffer. 1mg of protein was loaded per condition. Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with Trim27 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect TRIM27 signal.
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FH Sarah (Verified Customer) (07-03-2019) | Total cell lysate (30 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-TRIM27 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.
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FH Francesca (Verified Customer) (05-16-2019) | Excellent antibody, clean and strong signal.
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