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  • Featured Product
  • KD/KO Validated

TRIM21 Polyclonal antibody

TRIM21 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IP, coIP, ELISA

Conjugate

Unconjugated

Cat no : 12108-1-AP

Synonyms

RNF81, RNF 81, RING finger protein 81, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM21

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis using 12108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis using 12108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis of HeLa using 12108-1-AP

    WB result of TRIM21 antibody (12108-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM21 transfected HeLa cells.

  • Western Blot (WB) analysis of HL-60 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis of HL-60 using 12108-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis of A549 using 12108-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis of A549 using 12108-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis of HeLa using 12108-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    WB analysis of HT-1080 using 12108-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    IP experiment of A549 using 12108-1-AP

    IP result of anti-TRIM21 (IP:12108-1-AP, 4ug; Detection:12108-1-AP 1:500) with A549 cells lysate 920ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TRIM21 Polyclonal antibody (12108-1-AP)

    IHC staining of human pancreas cancer using 12108-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TRIM21 Polyclonal antibody (12108-1-AP)

    IHC staining of human pancreas cancer using 12108-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM21 Polyclonal antibody (12108-1-AP)

    IHC staining of human colon cancer using 12108-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM21 Polyclonal antibody (12108-1-AP)

    IHC staining of human colon cancer using 12108-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    IF Staining of HepG2 using 12108-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    IF Staining of A549 using 12108-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRIM21 Polyclonal antibody (12108-1-AP)

    IF Staining of A549 using 12108-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells, HL-60 cells, HT-1080 cells, HT-29 cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12108-1-AP targets TRIM21 in WB, IHC, IF/ICC, IP, coIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig, zebrafish, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM21 fusion protein Ag2753
Full Name tripartite motif-containing 21
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight 42-45 kDa, 49-54 kDa
GenBank Accession NumberBC010861
Gene Symbol TRIM21
Gene ID (NCBI) 6737
RRIDAB_2209469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIM21 antibody 12108-1-APDownload protocol
IHC protocol for TRIM21 antibody 12108-1-APDownload protocol
IF protocol for TRIM21 antibody 12108-1-APDownload protocol
IP protocol for TRIM21 antibody 12108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Clin Invest

The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain-of-function in cancer

Authors - Juan Liu
View Article
humanWB,IP

Adv Sci (Weinh)

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Authors - Qifan Hu
View Article
humanWB

Autophagy

Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules

Authors - Cuiwei Yang
  • KD Validated
View Article
humanWB,IP

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
View Article
humanIHC

Clin Transl Med

Chaperonin-containing TCP1 subunit 6A inhibition via TRIM21-mediated K48-linked ubiquitination suppresses triple-negative breast cancer progression through the AKT signalling pathway

Authors - Mengdi Yang
View Article
mouseIF

Transl Res

MECHANISM OF ACTION OF IC 100, A HUMANIZED IgG4 MONOCLONAL ANTIBODY TARGETING APOPTOSIS-ASSOCIATED SPECK-LIKE STAINING PROTEIN CONTAINING A CASPASE RECRUITMENT DOMAIN (ASC).

Authors - Juan Pablo de Rivero Vaccari
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Madison (Verified Customer) (05-28-2024)

The western blot showed very clear bands and had really precise staining. We got exactly what we wanted from the antibody on the first try

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rippa Insoluble treated mouse cell
TRIM21 Antibody Western Blot validation (1:500 dilution) in Rippa Insoluble treated mouse cell (Cat no:12108-1-AP)
FH

Paul (Verified Customer) (01-14-2020)

Used routinely for Western Blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEKs
FH

Laura (Verified Customer) (01-14-2020)

Gives a clean Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T
FH

Benjamin (Verified Customer) (01-10-2020)

Used many times in many WB and IF applications. Reliably antibody which detects TRIM21 basal as well as fusion proteins in WB and can be used to visualise TRIM21 in cells through IF.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500 WB, 1:200 IF
  • Cell Tissue Type: HEK, SHSY5Y
FH

Alinda (Verified Customer) (09-11-2019)

HEK were transfected with a TRIM21 overexpression vector- secondary anitbody used was rb Alexa 488.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: HEK-293T
TRIM21 Antibody Immunofluorescence, validation (1:200 dilution) in HEK-293T (Cat no:12108-1-AP)