TREM2 Polyclonal antibody
TREM2 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 27599-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
27599-1-AP targets TREM2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TREM2 fusion protein Ag26319 |
| Full Name | triggering receptor expressed on myeloid cells 2 |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 26-32 kDa |
| GenBank Accession Number | BC032362 |
| Gene Symbol | TREM2 |
| Gene ID (NCBI) | 54209 |
| RRID | AB_2880921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TREM2 antibody 27599-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study. | ||
Sci Adv TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma | ||
Front Immunol Identification of TREM2-positive tumor-associated macrophages in esophageal squamous cell carcinoma: implication for poor prognosis and immunotherapy modulation | ||
Nat Commun Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease | ||
Brain Res TREM2 regulates BV2 microglia activation and influences corticosterone-induced neuroinflammation in depressive disorders | ||
Cell Death Dis TREM2 deficiency impairs the energy metabolism of Schwann cells and exacerbates peripheral neurological deficits |
