TREM-1/CD354 Polyclonal antibody
TREM-1/CD354 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11791-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human peripheral blood leukocyte, human liver tissue, human spleen tissue, mouse lung tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 4 publications below |
| IF | See 10 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
11791-1-AP targets TREM-1/CD354 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TREM-1/CD354 fusion protein Ag2418 |
| Full Name | triggering receptor expressed on myeloid cells 1 |
| Calculated Molecular Weight | 234 aa, 26 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC017773 |
| Gene Symbol | TREM1 |
| Gene ID (NCBI) | 54210 |
| RRID | AB_2208556 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Triggering receptor expressed on myeloid cells 1 (TREM1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TREM-1/CD354 antibody 11791-1-AP | Download protocol |
| IHC protocol for TREM-1/CD354 antibody 11791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation. | ||
Int Immunopharmacol Activation of NLRP3 inflammasome up-regulates TREM-1 expression in murine macrophages via HMGB1 and IL-18. | ||
Acta Trop Malaria-derived exosomes exacerbate liver injury during blood stage of Plasmodium berghei infection | ||
J Inflamm Res Overexpression of TREM1 is Associated with the Immune-Suppressive Microenvironment and Unfavorable Prognosis in Pan-Cancer | ||
Neurol Neuroimmunol Neuroinflamm Jo-1 Antibodies From Myositis Induce Complement-Dependent Cytotoxicity and TREM-1 Upregulation in Muscle Endothelial Cells | ||
Behav Brain Res Inhibition of TREM-1 Ameliorates Lipopolysaccharide-induced Depressive-like Behaviors by Alleviating Neuroinflammation in the PFC via PI3K/Akt Signaling Pathway |
