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  • Featured Product
  • KD/KO Validated

TPI1 Polyclonal antibody

TPI1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10713-1-AP

Synonyms

Triose-phosphate isomerase, TPI, TIM, Methylglyoxal synthase, EC:5.3.1.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis using 10713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of HEK-293 using 10713-1-AP

    WB result of TPI1 antibody (10713-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of rat kidney tissue using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of rat kidney using 10713-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of RAW 264.7 using 10713-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of mouse liver using 10713-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of HEK-293 using 10713-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of mouse kidney using 10713-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of A431 using 10713-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of HepG2 using 10713-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using TPI1 Polyclonal antibody (10713-1-AP)

    WB analysis of rat liver using 10713-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TPI1 Polyclonal antibody (10713-1-AP)

    IP experiment of HEK-293 using 10713-1-AP

    IP result of anti-TPI1 (IP:10713-1-AP, 3ug; Detection:10713-1-AP 1:1000) with HEK-293 cells lysate 2500ug.

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10713-1-AP targets TPI1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, yeast, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPI1 fusion protein Ag1175
Full Name triosephosphate isomerase 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
RRIDAB_2207716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Protocols

Product Specific Protocols
WB protocol for TPI1 antibody 10713-1-APDownload protocol
IP protocol for TPI1 antibody 10713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.

Authors - Zhong Lei L
View Article

Nat Chem Biol

SF2312 is a natural phosphonate inhibitor of enolase.

Authors - Paul G Leonard
View Article
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
View Article
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
View Article
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
View Article
human,mouseWB,IHC,IF

Brain

Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation.

Authors - Guix Francesc X FX
View Article