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Tested Applications
| Positive WB detected in | L02 cells |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
20705-1-AP targets TOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | topoisomerase (DNA) I |
| Calculated Molecular Weight | 91 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | NM_003286 |
| Gene Symbol | TOP1 |
| Gene ID (NCBI) | 7150 |
| RRID | AB_10695762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11387 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOP1 antibody 20705-1-AP | Download protocol |
| IHC protocol for TOP1 antibody 20705-1-AP | Download protocol |
| IF protocol for TOP1 antibody 20705-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence. | ||
Cell Chem Biol Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons. | ||
J Transl Med Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1 | ||
Oncotarget Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study. | ||
J Cell Mol Med EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells. | ||
iScience Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype. |
