TOM20 Polyclonal antibody

IF on HeLa cells using PFA 4%, 37°C 1h, BSA 10% 30 min, and 1h Ab 1/1000 dilution in BSA 3% Correct mitochondrial network

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa cells

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 2000
• Cell Tissue Type: l2

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

Works well for immunofluorescence (1:200) and Western blotting (1:1000-1:2000)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human and mouse cancer cell lines

The antibody worked very well for immunofluorescence, combined with a secondary fluorescent antibody 555.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: COS-7

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: FIBROBLAST

This antibody works and provides robust signals of TOM20 immunoreactivity on feline tissue sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Optic nerve

We got very clear and correct bands for tom20., very good antibody.

• Applications: Western Blot

It is very specific and nicely labelled mitochondria. I highly suggest it. 4% paraformaldehyde fixation for 10 min at RT and 0.3% Triton-x100 10 minutes for permeabilization at RT. I used 2h at RT or 16h at 4 degree incubation. Alexa fluor 488 1:700 is used for 1 hour at RT for secondary ab.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

The tom 20 works great. We wanted to compare and it does stand out

• Applications: Immunohistochemistry

The antibody allows for exceptionally clear visualisation of the mitochondrial outer membrane. HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:250 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLa

TOM20 1:200 in PBST (0.1% triton) + 10% Donkey Serum O/N 4ºC incubation. Donkey anti-rabbit alexa fluor 555 1:500 1h RT incubation.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human fibroblasts

For mouse hippocampusWorks well

• Applications: Immunofluorescence,

TOM20 ab is great for ICC and WB. Tested in human primary fibroblats. ICC: fixed fibroblats with 4% PFA 15min. TOM20 diluted 1:200 in PBST (0.1% triton) + 10% DS O/N 4ºC incubation. Donkey anti-rabbit alexa fluor 488 1:500 1h RT. DAPI in blue.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human fibroblasts

Reliably used in western blot where it identifies the correct band with high specificity.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

WB worked fine

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEKs

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

It's great for IF.

• Applications: Immunofluorescence,
• Cell Tissue Type: Human

Good

• Applications: Western Blot,
• Cell Tissue Type: AC16

A very specific and reliable antibody. For Western Blot, it identifies the correct MW band in cellular and mitochondrial lysate from either murine (N2a) and human (HeLa and U2OS) cell. For immunofluorescence, it is ideal for localization studies: it works perfectly to label mitochondria instead of plasmidic markers.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: Western Blot 1:2000, Immunofluorescence 1:200
• Cell Tissue Type: N2a, U2OS and HeLa cells

SDS-PAGE: 15 ug protein/well.Lines on WB1. BioRad Precision Plus Protein standard2. Homogenate of mice brain tissue3. Non-synaptosomal mitochondria from mice brain.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mice brain tissue

It is an amazing antibody. Very specific and very bright at a 1:1000 dilution.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS cells