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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse testis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
18030-1-AP targets TNKS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNKS fusion protein Ag12597 Predict reactive species |
| Full Name | tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase |
| Calculated Molecular Weight | 1327 aa, 142 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC098394 |
| Gene Symbol | TNKS |
| Gene ID (NCBI) | 8658 |
| RRID | AB_2303564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95271 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNKS antibody 18030-1-AP | Download protocol |
| IHC protocol for TNKS antibody 18030-1-AP | Download protocol |
| IF protocol for TNKS antibody 18030-1-AP | Download protocol |
| IP protocol for TNKS antibody 18030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation | ||
Oncol Lett Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer. | ||
Cell Death Dis circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.
| ||
Chem Biol Interact Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer |
