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  • Featured Product
  • KD/KO Validated

TMED9 Polyclonal antibody

TMED9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21620-1-AP

Synonyms

p25, p24alpha2, p24 family protein alpha-2, GP25L2, GMP25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMED9 Polyclonal antibody (21620-1-AP)

    WB analysis using 21620-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TMED9 Polyclonal antibody (21620-1-AP)

    WB analysis of pig liver using 21620-1-AP

    pig liver tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using TMED9 Polyclonal antibody (21620-1-AP)

    WB analysis of mouse pancreas using 21620-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TMED9 Polyclonal antibody (21620-1-AP)

    WB analysis of mouse brain using 21620-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TMED9 Polyclonal antibody (21620-1-AP)

    WB analysis of mouse lung using 21620-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMED9 Polyclonal antibody (21620-1-AP)

    WB analysis using 21620-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TMED9 Polyclonal antibody (21620-1-AP)

    IP experiment of mouse liver using 21620-1-AP

    IP result of anti-TMED9 (IP:21620-1-AP, 4ug; Detection:21620-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMED9 Polyclonal antibody (21620-1-AP)

    IHC staining of human liver using 21620-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TMED9 Polyclonal antibody (21620-1-AP)

    IHC staining of human liver using 21620-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TMED9 Polyclonal antibody (21620-1-AP)

    IHC staining of human liver using 21620-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21620-1-AP (TMED9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMED9 Polyclonal antibody (21620-1-AP)

    IF Staining of HepG2 using 21620-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TMED9 Polyclonal antibody (21620-1-AP)

    IF Staining of HEK-293 using 21620-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21620-1-AP targets TMED9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMED9 fusion protein Ag13813
Full Name transmembrane emp24 protein transport domain containing 9
Calculated Molecular Weight 235 aa, 27 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC001123
Gene Symbol TMED9
Gene ID (NCBI) 54732
RRIDAB_10858623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

Protocols

Product Specific Protocols
WB protocol for TMED9 antibody 21620-1-APDownload protocol
IHC protocol for TMED9 antibody 21620-1-APDownload protocol
IF protocol for TMED9 antibody 21620-1-APDownload protocol
IP protocol for TMED9 antibody 21620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Authors - Moran Dvela-Levitt
View Article
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated
View Article
humanWB,CoIP

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated
View Article
humanWB

BMC Biol

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Authors - Xihua Yue
View Article
WB

Mol Biol Cell

The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes

Authors - Benjamin S Roberts
View Article
humanWB,IHC

Bioengineered

High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.

Authors - Gaoda Ju
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Giulia (Verified Customer) (02-29-2024)

Specific antibody that works well in Western blot, both for cells and tissue lysates.

  • Applications: Western Blot, Immunofluorescence