A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12909-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 12909-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human pancreas tissue, human testis tissue, mouse ovary tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A549 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of CHO cells using 12909-1-AP
WB result of 12909-1-AP from Takashi Kobayashi.
WB analysis of HeLa using 12909-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12909-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of rat ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of rat ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IF/ICC Figures
IF Staining of A549 using 12909-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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