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TFEB Polyclonal antibody
TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay
Conjugate
Unconjugated
198
Cat no : 13372-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells, rat brain tissue, HEK-293 cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 27 publications below |
| WB | See 165 publications below |
| IHC | See 18 publications below |
| IF | See 89 publications below |
| IP | See 6 publications below |
| CoIP | See 2 publications below |
Product Information
13372-1-AP targets TFEB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TFEB fusion protein Ag4194 |
| Full Name | transcription factor EB |
| Calculated Molecular Weight | 476 aa, 53 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC032448 |
| Gene Symbol | TFEB |
| Gene ID (NCBI) | 7942 |
| RRID | AB_2199611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFEB antibody 13372-1-AP | Download protocol |
| IHC protocol for TFEB antibody 13372-1-AP | Download protocol |
| IF protocol for TFEB antibody 13372-1-AP | Download protocol |
| IP protocol for TFEB antibody 13372-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes | ||
Autophagy SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Stanislas (Verified Customer) (08-05-2024) | WB on HeLa lysates using 15µg of protein and nitrocellulose membranes, blockage in TBST-5%Milk 1h, Incubation in 2%BSA with TFEB antibody (1:3000) overnight at 4°C. Worked on the first trial. IF on HeLa fixed in 4%PFA for 15min, blocked 1h in 1XPBS, 5%NGS, 0.3% Triton X-100, incubates with TFEB antibody (1:100) in 1XPBS, 1%BSA, 0.3% Triton X-100 overnight at 4°C. Worked on first trial.
|
FH Shunbin (Verified Customer) (07-17-2023) | bands look specific, but blurry and multiple.
 |
FH Weihao (Verified Customer) (04-11-2022) | Good antibody for mouse BMDM! not for human MDM 1:500 for IF 1:2000 for WB
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FH Marina (Verified Customer) (11-22-2021) | 2 bands visible, unclear which is the right one, although size is ok-ish for both. Both bands are quite weak.
|
FH Tom (Verified Customer) (02-19-2021) | HEK293 lysate (10ug) subjected to SDS-PAGE and transferred to nitrocellullose. Membrane blocked in 5% BSA for 1 hour prior to incubation with antibody (1:1000 in block) overnight at 4 degrees. Band detected using anti-rabbit HRP secondary.
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