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SYT7 Polyclonal antibody

SYT7 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 19741-1-AP

Synonyms

Synaptotagmin VII, Synaptotagmin 7, Prostate cancer-associated protein 7, PCANAP7, IPCA-7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYT7 Polyclonal antibody (19741-1-AP)

    WB analysis using 19741-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SYT7 Polyclonal antibody (19741-1-AP)

    IF Staining of mouse cerebellum using 19741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

19741-1-AP targets SYT7 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name synaptotagmin VII
Calculated Molecular Weight 46 kDa
Observed Molecular Weight42-45 kDa
GenBank Accession NumberNM_004200
Gene Symbol SYT7
Gene ID (NCBI) 9066
RRIDAB_3085583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Protocols

Product Specific Protocols
WB protocol for SYT7 antibody 19741-1-APDownload protocol
IF protocol for SYT7 antibody 19741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Brain Res Bull

Double-strand-break repair protein rad21 homolog/Synaptotagmin-7 alleviates Alzheimer's disease in mice by promoting M2 polarization of microglia

Authors - Cheng Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Olga (Verified Customer) (01-17-2025)

I was disapointed as it did not recognize the band of declared 42-46 kDa in any tissue and cell lysate tested - only the bands of about 55 kDa and mouse brain tissue and cells - and a band of about 100 kDa in all lanes

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (forebrain, liver, lung,heart)
SYT7 Antibody Western Blot validation (1:1000 dilution) in HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (forebrain, liver, lung,heart) (Cat no:19741-1-AP)