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CoraLite®594-conjugated STAT3 Monoclonal antibody
STAT3 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3G2D12
Cat no : CL594-60199
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL594-60199 is STAT3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | STAT3 fusion protein Ag0360 |
Full Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
Calculated Molecular Weight | 770 aa, 88 kDa |
Observed Molecular Weight | 88 kDa |
GenBank Accession Number | BC000627 |
Gene Symbol | STAT3 |
Gene ID (NCBI) | 6774 |
RRID | AB_2883438 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 STAT3 antibody CL594-60199 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |