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  • Featured Product
  • KD/KO Validated

SREBF1 Polyclonal antibody

SREBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14088-1-AP

Synonyms

SREBP 1c, SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis of HeLa using 14088-1-AP

    WB result of SREBF1 antibody (14088-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis of HeLa using 14088-1-AP

    WB result of SREBF1 antibody (14088-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis using 14088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis of L02 using 14088-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SREBF1 Polyclonal antibody (14088-1-AP)

    WB analysis of HeLa using 14088-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using SREBF1 Polyclonal antibody (14088-1-AP)

    IP experiment of L02 using 14088-1-AP

    IP result of anti-SREBF1 (IP:14088-1-AP, 4ug; Detection:14088-1-AP 1:600) with L02 cells lysate 1500ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SREBF1 Polyclonal antibody (14088-1-AP)

    IHC staining of human kidney using 14088-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SREBF1 Polyclonal antibody (14088-1-AP)

    IHC staining of human kidney using 14088-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SREBF1 Polyclonal antibody (14088-1-AP)

    IHC staining of human skeletal muscle using 14088-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SREBF1 Polyclonal antibody (14088-1-AP)

    IHC staining of human skeletal muscle using 14088-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:25 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SREBF1 Polyclonal antibody (14088-1-AP)

    IF Staining of HeLa using 14088-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14088-1-AP (SREBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14088-1-AP targets SREBF1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, bovine, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SREBF1 fusion protein Ag5219
Full Name sterol regulatory element binding transcription factor 1
Calculated Molecular Weight 1177 aa, 125 kDa
Observed Molecular Weight 125 kDa, 68 kDa
GenBank Accession NumberBC063281
Gene Symbol SREBF1
Gene ID (NCBI) 6720
RRIDAB_2255217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.

Protocols

Product Specific Protocols
WB protocol for SREBF1 antibody 14088-1-APDownload protocol
IHC protocol for SREBF1 antibody 14088-1-APDownload protocol
IF protocol for SREBF1 antibody 14088-1-APDownload protocol
IP protocol for SREBF1 antibody 14088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A metastasis map of human cancer cell lines.

Authors - Xin Jin
  • KO Validated
View Article
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
mouseIHC

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
View Article
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
View Article
mouseWB

Nat Commun

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Authors - Jinjie Duan
View Article
human

Hepatology

PMID: 29729186

Authors - Huixia Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Isha (Verified Customer) (05-18-2022)

Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IF. And for chIP 3ug/ml worked best.

  • Applications: Western Blot, Immunofluorescence, ChIP