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  • Featured Product
  • KD/KO Validated

SMARCA4/BRG1 Polyclonal antibody

SMARCA4/BRG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 21634-1-AP

Synonyms

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    WB analysis using 21634-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    WB analysis of HepG2 using 21634-1-AP

    WB result of SMARCA4/BRG1 antibody (21634-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    WB analysis using 21634-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    WB analysis of mouse brain using 21634-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    WB analysis of rat brain using 21634-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    WB analysis of human placenta using 21634-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IP experiment of HeLa using 21634-1-AP

    IP result of anti-SMARCA4/BRG1 (IP:21634-1-AP, 5ug; Detection:21634-1-AP 1:1000) with HeLa cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human colon cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human lung cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human gliomas using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human gliomas using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human lung cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human lung cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of mouse kidney using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of mouse kidney using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human breast cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human breast cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human colon cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IHC staining of human lung cancer using 21634-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IF Staining of HepG2 using 21634-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (21634-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IF Staining of HeLa using 21634-1-AP

    Immunofluorescent analysis of Hela cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMARCA4/BRG1 Polyclonal antibody (21634-1-AP)

    IF Staining of HEK-293 using 21634-1-AP

    Immunofluorescent analysis of 293 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue, MCF-7 cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse kidney tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21634-1-AP targets SMARCA4/BRG1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMARCA4/BRG1 fusion protein Ag16256
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated Molecular Weight 1647 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
RRIDAB_10858784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
WB protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
IHC protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
IF protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
IP protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Clin Oncol

Genomic Characterization of Non-Small-Cell Lung Cancer in African Americans by Targeted Massively Parallel Sequencing.

Authors - Luiz H Araujo
View Article
humanWB

Cell Stem Cell

The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling.

Authors - Yanying Wang
View Article
ChIP

Mol Cell

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Authors - Shibin Hu
View Article
humanWB,IP

Nucleic Acids Res

Reactivation of the G1 enhancer landscape underlies core circuitry addiction to SWI/SNF

Authors - Katerina Cermakova
View Article
humanWB,IHC

Nat Commun

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Authors - Pingping Zhu
View Article
mouseCoIP

Nucleic Acids Res

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Authors - Xu Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Satyendra (Verified Customer) (07-16-2021)

I have marked Knock out as KO

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293
SMARCA4/BRG1 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:21634-1-AP)