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Tested Applications
| Positive WB detected in | 37°C incubated HeLa cells, HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
14687-1-AP targets SLC44A1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SLC44A1 fusion protein Ag6024 Predict reactive species |
| Full Name | solute carrier family 44, member 1 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 70-73 kDa |
| GenBank Accession Number | BC049203 |
| Gene Symbol | SLC44A1 |
| Gene ID (NCBI) | 23446 |
| RRID | AB_10640573 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWI5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC44A1, also referred to as choline transporter-like protein 1 (CTL1) or CDw92, is a choline transporter detected in both the plasma and mitochondrial membranes where it transports choline at high affinity and in a Na+-independent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC44A1 antibody 14687-1-AP | Download protocol |
| IHC protocol for SLC44A1 antibody 14687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
Cell Prolif The nano-windmill exerts superior anti-inflammatory effects via reducing choline uptake to inhibit macrophage activation | ||
BMC Cancer Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy | ||
Cell Metab Transketolase promotes MAFLD by limiting inosine-induced mitochondrial activity | ||
