Filter: AllWBIHCIFFC (Intra)

WB analysis of mouse colon using 27759-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 27759-1-AP (SLC26A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat colon using 27759-1-AP
rat colon tissue were subjected to SDS PAGE followed by western blot with 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 27759-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27759-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27759-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 27759-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27759-1-AP (SLC26A2 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 27759-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLC26A2 antibody (27759-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of U2OS using 27759-1-AP
1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human SLC26A2 (27759-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SLC26A2 Polyclonal antibody

Name: SLC26A2 Polyclonal antibody
Brand: Proteintech
SKU: 27759-1-AP

SLC26A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 27759-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse colon tissue, rat colon tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 3 publications below

Product Information

27759-1-AP targets SLC26A2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC26A2 fusion protein Ag26521 Predict reactive species
Full Name	solute carrier family 26 (sulfate transporter), member 2
Calculated Molecular Weight	82 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC059390
Gene Symbol	SLC26A2
Gene ID (NCBI)	1836
RRID	AB_2880963
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50443
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC26A2 antibody 27759-1-AP	Download protocol
IHC protocol for SLC26A2 antibody 27759-1-AP	Download protocol
IF protocol for SLC26A2 antibody 27759-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse,canine	IF	Biomater Res ι-Carrageenan nanocomposites for enhanced stability and oral bioavailability of curcumin. Authors - Joo Young Lee View Article
human	IF	J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. Authors - Yalong Wang View Article
human	WB	iScience Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients Authors - Xiao-Hu Zhao View Article
human	IF	Cell Stem Cell Bioengineered human colon organoids with in vivo-like cellular complexity and function Authors - Olga Mitrofanova View Article