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SLC25A1 Polyclonal antibody

SLC25A1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15235-1-AP


CIC, Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NCI-H1299 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15235-1-AP targets SLC25A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A1 fusion protein Ag7352
Full Name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC004980
Gene Symbol SLC25A1
Gene ID (NCBI) 6576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)


Product Specific Protocols
WB protocol for SLC25A1 antibody 15235-1-APDownload protocol
IHC protocol for SLC25A1 antibody 15235-1-APDownload protocol
IF protocol for SLC25A1 antibody 15235-1-APDownload protocol
IP protocol for SLC25A1 antibody 15235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A non-canonical tricarboxylic acid cycle underlies cellular identity.

Authors - Paige K Arnold


Glycolytic Switch Is Required for Transdifferentiation to Endothelial Lineage.

Authors - Li Lai

Nat Commun

IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.

Authors - Weiwei Liu


Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer

Am J Hum Genet

Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.

Authors - Benjamin Nota

Cell Death Differ

Inhibition of the mitochondrial citrate carrier, Slc25a1, reverts steatosis, glucose intolerance, and inflammation in preclinical models of NAFLD/NASH.

Authors - Mingjun Tan


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Bruna (Verified Customer) (03-05-2022)

This ab works very well for WB in human and mouse cell lines. Very strong and specific band (tested with KO cells lines).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MEFs/U2OS

Chunling (Verified Customer) (12-06-2018)

This antibody works great for western blot and Immunofluorescence. It is very specific with no non specific bands in western.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: kidney cancer cells