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- KD/KO Validated
SLC20A2 Polyclonal antibody
SLC20A2 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 12820-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, COLO 320 cells, mouse kidney tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IF | See 1 publications below |
Product Information
12820-1-AP targets SLC20A2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SLC20A2 fusion protein Ag3469 |
| Full Name | solute carrier family 20 (phosphate transporter), member 2 |
| Calculated Molecular Weight | 652 aa, 70 kDa |
| Observed Molecular Weight | 68-70 kDa |
| GenBank Accession Number | BC028600 |
| Gene Symbol | SLC20A2 |
| Gene ID (NCBI) | 6575 |
| RRID | AB_2191004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC20A2 antibody 12820-1-AP | Download protocol |
| IHC protocol for SLC20A2 antibody 12820-1-AP | Download protocol |
| IP protocol for SLC20A2 antibody 12820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds
| ||
Br J Pharmacol Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease. | ||
J Biol Chem Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake. | ||
Arterioscler Thromb Vasc Biol Potential Role of H-Ferritin in Mitigating Valvular Mineralization. | ||
Mov Disord A Homozygous Variant in NAA60 Is Associated with Primary Familial Brain Calcification |
