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  • Western Blot (WB) analysis of various lysates using SKP1 Monoclonal antibody (67745-1-Ig)

    WB analysis using 67745-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67745-1-Ig (SKP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SKP1 Monoclonal antibody (67745-1-Ig)

    IF Staining of MCF-7 using 67745-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SKP1 antibody (67745-1-Ig, Clone: 1D5G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SKP1 Monoclonal antibody

SKP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67745-1-Ig
Clone No.1D5G4

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EMC19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67745-1-Ig targets SKP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SKP1 fusion protein Ag30709 Predict reactive species
Full Name S-phase kinase-associated protein 1
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-20 kDa
GenBank Accession NumberBC009839
Gene Symbol SKP1
Gene ID (NCBI) 6500
RRIDAB_2918514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63208
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Protocols

Product Specific Protocols
WB protocol for SKP1 antibody 67745-1-IgDownload protocol
IF protocol for SKP1 antibody 67745-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols