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  • KD/KO Validated

Snf1lk Polyclonal antibody

Snf1lk Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 51045-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Sik1, Salt-inducible kinase 1, salt inducible kinase 1, Myocardial SNF1-like kinase, Msk

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1000 μg/ml
20 μL, 1000 μg/ml
150 μL, 1000 μg/ml

$449/ 150 μL

Freight/Packing: $40.00

Quantity

Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.


Proteintech Guarantee



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51045-1-AP targets Snf1lk in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Snf1lk fusion protein Ag3913 Predict reactive species
Full Name salt inducible kinase 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85-100 kDa
GenBank Accession NumberNM_010831
Gene Symbol Sik1
Gene ID (NCBI) 17691
RRIDAB_2187322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57059
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and the expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).

Protocols

Product Specific Protocols
WB protocol for Snf1lk antibody 51045-1-APDownload protocol
IHC protocol for Snf1lk antibody 51045-1-APDownload protocol
IF protocol for Snf1lk antibody 51045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Phytomedicine

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis

Authors - Daofei Song
humanWB,IHC

iScience

Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus

Authors - Jie Ning
  • KD Validated
humanWB, IF

J Neuroinflammation

High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.

Authors - Mengyan Hu
humanWB

J Cell Physiol

ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes

Authors - Xueyi Chen
mouse,ratIHC,IF

J Cereb Blood Flow Metab

Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.

Authors - Cheng Jian J
humanWB

J Leukoc Biol

SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.

Authors - Maria Stella Lombardi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

SD (Verified Customer) (07-05-2024)

Antibody worked well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: A549
Snf1lk Antibody Western Blot validation (1000 dilution) in A549 (Cat no:51045-1-AP)
FH

P (Verified Customer) (05-16-2024)

Good

  • Applications: Western Blot
  • Primary Antibody Dilution: 500
  • Cell Tissue Type: AC16
Snf1lk Antibody Western Blot validation (500 dilution) in AC16 (Cat no:51045-1-AP)
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