mouse brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of chicken brain using 67841-1-Ig
chicken brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
chicken brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67841-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rabbit brain using 67841-1-Ig
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of chicken brain using 67841-1-Ig
chicken brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67841-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rabbit brain using 67841-1-Ig
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SH3BP5 Monoclonal antibody
Catalog Number
67841-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000
Applications
WB, ELISA
Reactivity
human, rabbit, mouse, rat, pig, chicken
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
