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SF1 Polyclonal antibody

SF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Cat no : 11635-1-AP

Synonyms

BBP, D11S636, mBBP, SF1, splicing factor 1, Transcription factor ZFM1, ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SF1 Polyclonal antibody (11635-1-AP)

    WB analysis using 11635-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using SF1 Polyclonal antibody (11635-1-AP)

    IHC staining of mouse ovary using 11635-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using SF1 Polyclonal antibody (11635-1-AP)

    IHC staining of mouse ovary using 11635-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11635-1-AP targets SF1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF1 fusion protein Ag2209
Full Name splicing factor 1
Calculated Molecular Weight 638 aa, 68 kDa
Observed Molecular Weight65-71 kDa
GenBank Accession NumberBC020217
Gene Symbol SF1
Gene ID (NCBI) 7536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SF1 antibody 11635-1-APDownload protocol
IHC protocol for SF1 antibody 11635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Retrovirology

A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.

Authors - Rebecca Moschall
View Article
humanIHC,IF

Cancers (Basel)

Targeting Oncogenic Wnt/β-Catenin Signaling in Adrenocortical Carcinoma Disrupts ECM Expression and Impairs Tumor Growth

Authors - Morgan K Penny
View Article
humanIF

Front Endocrinol (Lausanne)

Pharmacogenomic analysis in adrenocortical carcinoma reveals genetic features associated with mitotane sensitivity and potential therapeutics

Authors - Jie Zhang
View Article