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- KD/KO Validated
Septin 7 Polyclonal antibody
Septin 7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, CoIP, ELISA, PLA
Conjugate
Unconjugated
Cat no : 13818-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HepG2 cells, Jurkat cells, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human gliomas tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 18 publications below |
| IF | See 18 publications below |
| CoIP | See 1 publications below |
Product Information
13818-1-AP targets Septin 7 in WB, IHC, IF/ICC, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Septin 7 fusion protein Ag4907 |
| Full Name | septin 7 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC025987 |
| Gene Symbol | Septin 7 |
| Gene ID (NCBI) | 989 |
| RRID | AB_2254298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 7 antibody 13818-1-AP | Download protocol |
| IHC protocol for Septin 7 antibody 13818-1-AP | Download protocol |
| IF protocol for Septin 7 antibody 13818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol The septin cytoskeleton regulates natural killer cell lytic granule release.
| ||
Cell Rep Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts. | ||
MBio Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.
| ||
Development Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Amaury (Verified Customer) (03-29-2022) | I performed immunolabelling on K562. Used ice-cold methanol to permeabilize the cells and obtain very nice labelling
 |
FH stephen (Verified Customer) (02-19-2019) | HeLa cells fixed with 4%PFA and permblised with 0.3% triton x100 for 5 mins. stained with septin 7ab 1:300. (green) and DAPI to mark DNA (blue). non dividing interphase cell (top). Dividing cell in anaphase (bottom)
 |
