• Featured Product
  • KD/KO Validated

Septin 7 Polyclonal antibody

Septin 7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA, PLA

Conjugate

Unconjugated

Cat no : 13818-1-AP

Synonyms

septin7, SEPT7A, SEPT7, SEPT 7, CDC3



Tested Applications

Positive WB detected inA375 cells, HepG2 cells, Jurkat cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13818-1-AP targets Septin 7 in WB, IHC, IF/ICC, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 7 fusion protein Ag4907
Full Name septin 7
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC025987
Gene Symbol Septin 7
Gene ID (NCBI) 989
RRIDAB_2254298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

Protocols

Product Specific Protocols
WB protocol for Septin 7 antibody 13818-1-APDownload protocol
IHC protocol for Septin 7 antibody 13818-1-APDownload protocol
IF protocol for Septin 7 antibody 13818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Still and rotating myosin clusters determine cytokinetic ring constriction.

Authors - Viktoria Wollrab
humanWB

J Cell Biol

The septin cytoskeleton regulates natural killer cell lytic granule release.

Authors - Prasad V Phatarpekar
  • KD Validated
mouseWB,IF

Cell Rep

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Authors - Fernando Calvo
mouseIF,PLA

EMBO Rep

Cdc42-Borg4-Septin7 axis regulates HSC polarity and function

Authors - Ravinder Kandi
humanWB,IF

MBio

Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.

Authors - Quynh T Phan
  • KD Validated
bovineIF

Development

Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling.

Authors - Mengyun Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Amaury (Verified Customer) (03-29-2022)

I performed immunolabelling on K562. Used ice-cold methanol to permeabilize the cells and obtain very nice labelling

  • Applications: Immunofluorescence
  • Cell Tissue Type: K562
Septin 7 Antibody Immunofluorescence validation ( dilution) in K562 (Cat no:13818-1-AP)
FH

stephen (Verified Customer) (02-19-2019)

HeLa cells fixed with 4%PFA and permblised with 0.3% triton x100 for 5 mins. stained with septin 7ab 1:300. (green) and DAPI to mark DNA (blue). non dividing interphase cell (top). Dividing cell in anaphase (bottom)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HeLa cells
Septin 7 Antibody Immunofluorescence, validation (1:300 dilution) in HeLa cells (Cat no:13818-1-AP)