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SEMA4A Polyclonal antibody

SEMA4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27359-1-AP

Synonyms

CORD10, RP35, Sema B, SEMA4A, SEMAB, Semaphorin 4A, Semaphorin B, SEMB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA4A Polyclonal antibody (27359-1-AP)

    WB analysis using 27359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27359-1-AP (SEMA4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SEMA4A Polyclonal antibody (27359-1-AP)

    WB analysis of HeLa using 27359-1-AP

    WB result of SEMA4A antibody (27359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEMA4A transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SEMA4A Polyclonal antibody (27359-1-AP)

    IHC staining of human breast cancer using 27359-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SEMA4A Polyclonal antibody (27359-1-AP)

    IHC staining of human breast cancer using 27359-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SEMA4A Polyclonal antibody (27359-1-AP)

    IHC staining of human placenta using 27359-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SEMA4A Polyclonal antibody (27359-1-AP)

    IHC staining of human placenta using 27359-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27359-1-AP targets SEMA4A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEMA4A fusion protein Ag26023
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Calculated Molecular Weight 761 aa, 84 kDa
Observed Molecular Weight 120-150 kDa
GenBank Accession NumberBC020974
Gene Symbol SEMA4A
Gene ID (NCBI) 64218
RRIDAB_2880854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sema4A, the membrane-bound class 4 semaphorin, belongs to semaphorins superfamily. Sema4A funtions as potent axonal repellants in the nervous system, and is involved in vascular development. Two species of Sema4A with molecular weights of about 150 and 120 kDa were observed. The antibody recognizes the Sema4A protein, which is of appropriate molecular size (~120 kDa) for the glycosylated protein(PMID: 26296689/17318185/26024919).

Protocols

Product Specific Protocols
WB protocol for SEMA4A antibody 27359-1-APDownload protocol
IHC protocol for SEMA4A antibody 27359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

SEMA4A promotes prostate cancer invasion: involvement of tumor microenvironment

Authors - Xiao Liu
View Article
humanWB,IHC

Biomolecules

Semaphorin 4A Maintains Trophoblastic Function via Activating the STAT3 Pathway

Authors - Taotao Hou
View Article