P-selectin / CD62P Polyclonal antibody
P-selectin / CD62P Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13304-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human peripheral blood platelets |
| Positive IHC detected in | human spleen tissue, human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 2 publications below |
| FC | See 2 publications below |
Product Information
13304-1-AP targets P-selectin / CD62P in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | P-selectin / CD62P fusion protein Ag4124 |
| Full Name | selectin P (granule membrane protein 140kDa, antigen CD62) |
| Calculated Molecular Weight | 830 aa, 91 kDa |
| GenBank Accession Number | BC028067 |
| Gene Symbol | CD62P |
| Gene ID (NCBI) | 6403 |
| RRID | AB_2877937 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P-selectin / CD62P antibody 13304-1-AP | Download protocol |
| IHC protocol for P-selectin / CD62P antibody 13304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces AIEgen-Based Bionic Nanozymes for the Interventional Photodynamic Therapy-Based Treatment of Orthotopic Colon Cancer. | ||
ACS Appl Mater Interfaces Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression | ||
Int J Biol Macromol Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing | ||
iScience Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis | ||
World J Gastroenterol Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer | ||
Immun Inflamm Dis Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis. |
