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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55204-1-AP targets S1PR3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | sphingosine-1-phosphate receptor 3 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | NM_005226 |
| Gene Symbol | S1PR3 |
| Gene ID (NCBI) | 1903 |
| RRID | AB_3670241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99500 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
S1PR3 (sphingosine-1-phosphate receptor 3), also known as as S1P3 or EDG3, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR3 has a role in the development of a stable and mature vascular system during embryonic development (PMID: 15138255).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S1PR3 antibody 55204-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
