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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
15138-1-AP targets RPAP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPAP1 fusion protein Ag7286 Predict reactive species |
| Full Name | RNA polymerase II associated protein 1 |
| Calculated Molecular Weight | 153 kDa |
| Observed Molecular Weight | 153 kDa |
| GenBank Accession Number | BC000246 |
| Gene Symbol | RPAP1 |
| Gene ID (NCBI) | 26015 |
| RRID | AB_2301137 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWH6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPAP1 antibody 15138-1-AP | Download protocol |
| IP protocol for RPAP1 antibody 15138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.
| ||
Cell Rep Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome. |
