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RPA1 Polyclonal antibody

RPA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12448-1-AP

Synonyms

HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RPA1 Polyclonal antibody (12448-1-AP)

    WB analysis using 12448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RPA1 Polyclonal antibody (12448-1-AP)

    WB analysis of Y79 using 12448-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPA1 Polyclonal antibody (12448-1-AP)

    WB analysis using 12448-1-AP

    K-562 cell lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using RPA1 Polyclonal antibody (12448-1-AP)

    WB analysis of A431 using 12448-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RPA1 Polyclonal antibody (12448-1-AP)

    WB analysis of Y79 using 12448-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, Y79 cells, A431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12448-1-AP targets RPA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPA1 fusion protein Ag3067
Full Name replication protein A1, 70kDa
Calculated Molecular Weight 616 aa, 68 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC018126
Gene Symbol RPA1
Gene ID (NCBI) 6117
RRIDAB_2180495
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Replication protein A 1 (RPA1) is a subunit of a stable heterotrimer replication protein A (RPA), which is conserved in almost all eukaryotic species (PMID: 27151292). Usually, RPA1, RPA2 and RPA3 function as a whole to participate in DNA replication, telomere maintenance, DNA recombination, DNA repair, and activation of DNA damage checkpoints. In the cell, RPA has a higher affinity to bind single strand DNA and then to form a fork protection complex with other components such as PTEN and OTUB1, thus maintains genome stability during replication(PMID: 26403191; PMID: 18469000).

Protocols

Product Specific Protocols
WB protocol for RPA1 antibody 12448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

PMID: 31249811

Authors - Ma-Sha Huang
View Article