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RNASEH1 Polyclonal antibody
RNASEH1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 15606-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, U2OS cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 30 publications below |
IHC | See 1 publications below |
IF | See 17 publications below |
ChIP | See 1 publications below |
Product Information
15606-1-AP targets RNASEH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RNASEH1 fusion protein Ag7990 |
Full Name | ribonuclease H1 |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | BC002973 |
Gene Symbol | RNASEH1 |
Gene ID (NCBI) | 246243 |
RRID | AB_2238624 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
IF protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
IP protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. | ||
Cell Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy. | ||
Nature BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
| ||
Cell Res Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. | ||
Nat Cell Biol Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. | ||
Mol Cell METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mulavini (Verified Customer) (09-23-2024) | Works good
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FH R (Verified Customer) (06-15-2024) | Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells
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FH Andrea (Verified Customer) (10-05-2023) | Strong signal, very good detection.
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FH Huai-Chin (Verified Customer) (06-08-2019) | Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.
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