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RNASE3 Polyclonal antibody

RNASE3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55338-1-AP

Synonyms

ECP, Eosinophil cationic protein, Ribonuclease 3, RNase 3, RNASE3, RNS3

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RNASE3 Polyclonal antibody (55338-1-AP)

    WB analysis of A549 using 55338-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55338-1-AP (RNASE3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RNASE3 Polyclonal antibody (55338-1-AP)

    WB analysis of HepG2 using 55338-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55338-1-AP (RNASE3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNASE3 Polyclonal antibody (55338-1-AP)

    IHC staining of human lung cancer using 55338-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55338-1-AP (RNASE3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNASE3 Polyclonal antibody (55338-1-AP)

    IHC staining of human lung cancer using 55338-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55338-1-AP (RNASE3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNASE3 Polyclonal antibody (55338-1-AP)

    IF Staining of HepG2 using 55338-1-AP

    Immunofluorescent analysis of HepG2 cells using 55338-1-AP (RNASE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55338-1-AP targets RNASE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ribonuclease, RNase A family, 3 (eosinophil cationic protein)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberNM_002935
Gene Symbol RNASE3
Gene ID (NCBI) 6037
RRIDAB_2881312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNASE3 antibody 55338-1-APDownload protocol
IHC protocol for RNASE3 antibody 55338-1-APDownload protocol
IF protocol for RNASE3 antibody 55338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Allergy

Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity

Authors - Jonathan G Medernach
View Article
mouseIF

Cell Commun Signal

Allergen-induced CD11c + dendritic cell pyroptosis aggravates allergic rhinitis

Authors - Yue-Long Qiao
View Article
mouseIHC

J Pharm Biomed Anal

Network pharmacology analysis and experimental validation of Xiao-Qing-Long-Tang's therapeutic effects against neutrophilic asthma

Authors - Zhifeng Chen
View Article