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- KD/KO Validated
RMI1 Polyclonal antibody
RMI1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14630-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
14630-1-AP targets RMI1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RMI1 fusion protein Ag6208 |
| Full Name | RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC064937 |
| Gene Symbol | RMI1 |
| Gene ID (NCBI) | 80010 |
| RRID | AB_2301203 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RMI1 antibody 14630-1-AP | Download protocol |
| IHC protocol for RMI1 antibody 14630-1-AP | Download protocol |
| IP protocol for RMI1 antibody 14630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
| ||
Nat Commun Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia.
| ||
J Biol Chem DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA-end resection in human cells.
| ||
J Cancer Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p. | ||
DNA Cell Biol Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure. | ||
Int J Mol Sci Accumulation and Phosphorylation of RecQ-Mediated Genome Instability Protein 1 (RMI1) at Serine 284 and Serine 292 during Mitosis. |
