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  • KD/KO Validated

RMI1 Polyclonal antibody

RMI1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14630-1-AP

Synonyms

BLAP75, C9orf76, FAAP75, RMI1, RP11 346I8.1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14630-1-AP targets RMI1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RMI1 fusion protein Ag6208
Full Name RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae)
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC064937
Gene Symbol RMI1
Gene ID (NCBI) 80010
RRIDAB_2301203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)

Protocols

Product Specific Protocols
WB protocol for RMI1 antibody 14630-1-APDownload protocol
IHC protocol for RMI1 antibody 14630-1-APDownload protocol
IP protocol for RMI1 antibody 14630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.

Authors - Thomas J Nicholls
  • KD Validated
humanWB

Nat Commun

Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia.

Authors - Martin Moder
  • KO Validated
humanWB

J Biol Chem

DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA-end resection in human cells.

Authors - Andreas Sturzenegger
  • KD Validated
humanWB

J Cancer

Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p.

Authors - Jin Qian
humanWB

DNA Cell Biol

Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure.

Authors - Yuxiao Sun
humanWB

Int J Mol Sci

Accumulation and Phosphorylation of RecQ-Mediated Genome Instability Protein 1 (RMI1) at Serine 284 and Serine 292 during Mitosis.

Authors - Chang Xu