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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
19556-1-AP targets RGC32 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | chromosome 13 open reading frame 15 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 13-15 kDa |
| GenBank Accession Number | NM_014059 |
| Gene Symbol | RGC32 |
| Gene ID (NCBI) | 28984 |
| RRID | AB_3085580 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4X1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RGC32, also named as C13orf15, modulates the activity of cell cycle-specific kinases. RGC32 enhances CDC2 activity. RGC32 may contribute to the regulation of the cell cycle. And it inhibits growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. The antibody can recognize these 2 isoforms of RGC32.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGC32 antibody 19556-1-AP | Download protocol |
| IHC protocol for RGC32 antibody 19556-1-AP | Download protocol |
| IF protocol for RGC32 antibody 19556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol RGC32 Promotes Bleomycin-Induced Systemic Sclerosis in a Murine Disease Model by Modulating Classically Activated Macrophage Function.
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