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RFC4 Polyclonal antibody

RFC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10806-1-AP

Synonyms

RFC37, RF-C 37 kDa subunit, A1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RFC4 Polyclonal antibody (10806-1-AP)

    WB analysis of MCF-7 using 10806-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RFC4 Polyclonal antibody (10806-1-AP)

    WB analysis of Raji using 10806-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RFC4 Polyclonal antibody (10806-1-AP)

    WB analysis of MCF-7 using 10806-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RFC4 Polyclonal antibody (10806-1-AP)

    WB analysis of Raji using 10806-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using RFC4 Polyclonal antibody (10806-1-AP)

    WB analysis of LNCaP using 10806-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RFC4 Polyclonal antibody (10806-1-AP)

    WB analysis of Raji using 10806-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RFC4 Polyclonal antibody (10806-1-AP)

    IHC staining of human skin cancer using 10806-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10806-1-AP (RFC4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RFC4 Polyclonal antibody (10806-1-AP)

    IHC staining of human skin cancer using 10806-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10806-1-AP (RFC4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RFC4 Polyclonal antibody (10806-1-AP)

    IF Staining of U2OS using 10806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RFC4 Polyclonal antibody (10806-1-AP)

    IF Staining of U2OS using 10806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RFC4 Polyclonal antibody (10806-1-AP)

    IF Staining of HepG2 using 10806-1-AP

    Immunofluorescent analysis of HepG2 cells, using RFC4 antibody 10806-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inMCF-7 cells, LNCaP cells, Raji cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

10806-1-AP targets RFC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFC4 fusion protein Ag1234
Full Name replication factor C (activator 1) 4, 37kDa
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC017452
Gene Symbol RFC4
Gene ID (NCBI) 5984
RRIDAB_2178477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RFC4 antibody 10806-1-APDownload protocol
IHC protocol for RFC4 antibody 10806-1-APDownload protocol
IF protocol for RFC4 antibody 10806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.

Authors - Jing Wang
View Article
mouseWB

Cell Rep

Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.

Authors - Stephen Gray
View Article
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang
View Article