Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22335-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 22335-1-AP
IP result of anti-RCC1 (IP:22335-1-AP, 3ug; Detection:22335-1-AP 1:1500) with HeLa cells lysate 3000ug.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human small intestine using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 22335-1-AP
Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
SH-SY5Y cells, HepG2 cells, mouse colon tissue, rat colon tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
mouse testis tissue, human small intestine tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:5000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against full length RCC1 of human origin.
Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22335-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse testis using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human small intestine using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 22335-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of HeLa using 22335-1-AP
IP result of anti-RCC1 (IP:22335-1-AP, 3ug; Detection:22335-1-AP 1:1500) with HeLa cells lysate 3000ug.
IF/ICC Figures
IF Staining of HepG2 using 22335-1-AP
Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RCC1 Polyclonal antibody
Catalog Number
22335-1-AP
Citations
1
Dilutions
WB : 1:1000-1:5000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
