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  • KD/KO Validated

RBP1 Polyclonal antibody

RBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22683-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

CRBPI, CRBP1, CRBP I, CRABP I, Cellular retinol-binding protein I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, human brain tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22683-1-AP targets RBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBP1 fusion protein Ag18572 Predict reactive species
Full Name retinol binding protein 1, cellular
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight 14-21 kDa
GenBank Accession NumberBC121052
Gene Symbol RBP1
Gene ID (NCBI) 5947
RRIDAB_11182381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09455
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBP1 antibody 22683-1-APDownload protocol
IHC protocol for RBP1 antibody 22683-1-APDownload protocol
IF protocol for RBP1 antibody 22683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Metabolism

Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.

Authors - Shyi-Jang Shin
humanWB,IHC

Transl Oncol

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Authors - Xinlu Liu
ratIHC

Brain Sci

Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus.

Authors - Tianjia Li
mouseWB

J Food Biochem

Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

Authors - Wei Li
humanWB,IHC,IF

Cell Death Dis

The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.

Authors - Ling Gao
  • KD Validated
humanWB

FASEB J

The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation

Authors - Dan-Dan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Melissa (Verified Customer) (06-13-2022)

We have used the antibody and it worked out

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1
  • Cell Tissue Type: Endothelial cells
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