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WB analysis using 22683-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22683-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 22683-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 22683-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 22683-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 22683-1-AP (RBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RBP1 Polyclonal antibody

Name: RBP1 Polyclonal antibody
Brand: Proteintech
SKU: 22683-1-AP
Rating: 5.00 (1 reviews)

RBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22683-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CRBPI, CRBP1, CRBP I, CRABP I, Cellular retinol-binding protein I

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (8)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse liver tissue, HEK-293 cells, human brain tissue, human liver tissue, rat liver tissue
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, human liver tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

22683-1-AP targets RBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RBP1 fusion protein Ag18572 Predict reactive species
Full Name	retinol binding protein 1, cellular
Calculated Molecular Weight	135 aa, 16 kDa
Observed Molecular Weight	14-21 kDa
GenBank Accession Number	BC121052
Gene Symbol	RBP1
Gene ID (NCBI)	5947
RRID	AB_11182381
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09455
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBP1 antibody 22683-1-AP	Download protocol
IHC protocol for RBP1 antibody 22683-1-AP	Download protocol
IF protocol for RBP1 antibody 22683-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Metabolism Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved. Authors - Shyi-Jang Shin View Article
human	WB,IHC	Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. Authors - Xinlu Liu View Article
rat	IHC	Brain Sci Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus. Authors - Tianjia Li View Article
mouse	WB	J Food Biochem Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice. Authors - Wei Li View Article
human	WB,IHC,IF	Cell Death Dis The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma. Authors - Ling Gao KD Validated View Article
human	WB	FASEB J The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation Authors - Dan-Dan Chen View Article