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WB analysis of HeLa using 17755-1-AP
WB result of RANBP9 antibody (17755-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP9 transfected HeLa cells.
WB analysis using 17755-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17755-1-AP (RANBP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17755-1-AP
IP result of anti-RANBP9 (IP:17755-1-AP, 4ug; Detection:17755-1-AP 1:600) with HeLa cells lysate 1600ug.
IHC staining of human testis using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse testis using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse testis using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of rat testis using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of rat testis using 17755-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MDCK using 17755-1-AP
Immunofluorescent analysis of MDCK cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 17755-1-AP
Immunofluorescent analysis of HeLa cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RANBP9 Polyclonal antibody

Name: RANBP9 Polyclonal antibody
Brand: Proteintech
SKU: 17755-1-AP

RANBP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17755-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

BPM L, BPM90, RAN binding protein 9, Ran binding protein M, RanBP7, RANBP9, RANBPM

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, mouse testis
Positive IP detected in	HeLa cells
Positive IHC detected in	human testis tissue, human skin tissue, mouse testis tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MDCK cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

17755-1-AP targets RANBP9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity	human, mouse, rat, Canine
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RANBP9 fusion protein Ag12112 Predict reactive species
Full Name	RAN binding protein 9
Calculated Molecular Weight	388aa,43 kDa; 729aa,78 kDa
Observed Molecular Weight	80-90 kDa
GenBank Accession Number	BC063849
Gene Symbol	RANBP9
Gene ID (NCBI)	10048
RRID	AB_2176803
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96S59
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RANBP9 antibody 17755-1-AP	Download protocol
IHC protocol for RANBP9 antibody 17755-1-AP	Download protocol
IF protocol for RANBP9 antibody 17755-1-AP	Download protocol
IP protocol for RANBP9 antibody 17755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	mSphere B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition. Authors - Yan Chen View Article
human	WB	Oncol Lett RANBP9 suppresses tumor proliferation in colorectal cancer. Authors - Chunzhi Qin View Article
rat	IF	Acta Histochem MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9. Authors - Huiyuan Zhang View Article
human	IHC	Transl Cancer Res Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients. Authors - Guangbin Wu View Article
mouse	WB	Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth Authors - Nelson V Simwela View Article