Tested Applications
| Positive WB detected in | HeLa cells, mouse spleen tissue, K-562 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11726-2-AP targets RAD1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rainbow trout |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2316 Product name: Recombinant human RAD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC006837 Sequence: MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES Predict reactive species |
| Full Name | RAD1 homolog (S. pombe) |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC006837 |
| Gene Symbol | RAD1 |
| Gene ID (NCBI) | 5810 |
| RRID | AB_2246457 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAD1, cell cycle checkpoint protein, is a component of a heterotrimeric cell cycle checkpoint 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. RAD1, HUS1 and RAD9 are central components of a DNA damage-responsive protein complex. Testis-specific disruption of RAD1 in mice resulted in defective double-strand break repair, depletion of germ cells and infertility (PMID: 35133274). RAD1 has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAD1 antibody 11726-2-AP | Download protocol |
| IHC protocol for RAD1 antibody 11726-2-AP | Download protocol |
| IP protocol for RAD1 antibody 11726-2-AP | Download protocol |
| WB protocol for RAD1 antibody 11726-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The affinity-purified RAD1 antibody (Cat# 11726-2-AP) has 7 total citations published in Drug Resist Updat, Phytomedicine, Oncogene, Mol Med, J Mol Cell Biol, Am J Physiol Cell Physiol, and Comp Biochem Physiol C Toxicol Pharmacol. Research fields include DNA damage response, gastric cancer drug resistance, pyroptosis in lung adenocarcinoma, homologous recombination, cell cycle regulation, genome stability maintenance, and RAD1 gene conservation in rainbow trout.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat RAD1 promotes oxaliplatin resistance in gastric cancer by reinforcing NRF2-driven antioxidant defense and DDR checkpoint signaling. | ||
Phytomedicine Baohuoside I induces GSDME-dependent pyroptosis and synergistically inhibits lung adenocarcinoma with cisplatin. | ||
Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity. | ||
Mol Med Rad1 attenuates DNA double-strand breaks and cell cycle arrest in type II alveolar epithelial cells of rats with bronchopulmonary dysplasia | ||
J Mol Cell Biol SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress. | ||
Am J Physiol Cell Physiol Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability. |
