|Positive WB detected in||HeLa cells, L02 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11726-2-AP targets RAD1 in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rainbow trout|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAD1 fusion protein Ag2316|
|Full Name||RAD1 homolog (S. pombe)|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC006837|
|Gene ID (NCBI)||5810|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for RAD1 antibody 11726-2-AP||Download protocol|
|IHC protocol for RAD1 antibody 11726-2-AP||Download protocol|
|IF protocol for RAD1 antibody 11726-2-AP||Download protocol|
|IP protocol for RAD1 antibody 11726-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Comp Biochem Physiol C Toxicol Pharmacol
The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.
Am J Physiol Cell Physiol
Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.
J Mol Cell Biol
SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.
DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.