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  • Featured Product
  • KD/KO Validated

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17146-1-AP

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PYCR2 Polyclonal antibody (17146-1-AP)

    WB analysis using 17146-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PYCR2 Polyclonal antibody (17146-1-AP)

    WB analysis of L02 using 17146-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PYCR2 Polyclonal antibody (17146-1-AP)

    WB analysis of Jurkat using 17146-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PYCR2 Polyclonal antibody (17146-1-AP)

    WB analysis of Jurkat using 17146-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PYCR2 Polyclonal antibody (17146-1-AP)

    WB analysis of HeLa using 17146-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human breast cancer using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human breast cancer using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human placenta using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human placenta using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human lung using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human lung using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human kidney using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human kidney using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human testis using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PYCR2 Polyclonal antibody (17146-1-AP)

    IHC staining of human testis using 17146-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PYCR2 Polyclonal antibody (17146-1-AP)

    IF Staining of HeLa using 17146-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, L02 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17146-1-AP targets PYCR2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, African green monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR2 fusion protein Ag10918
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated Molecular Weight 320 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC020553
Gene Symbol PYCR2
Gene ID (NCBI) 29920
RRIDAB_2253344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR2 antibody 17146-1-APDownload protocol
IHC protocol for PYCR2 antibody 17146-1-APDownload protocol
IF protocol for PYCR2 antibody 17146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Authors - Qiang Gao
View Article
humanWB

Sci Adv

Loss of attachment promotes proline accumulation and excretion in cancer cells

Authors - Steven E Pilley
View Article
mouseWB

EMBO J

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer
View Article
humanWB

EMBO J

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.

Authors - Zhen Chen
View Article
humanWB

Cell Rep

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

Authors - Kate E R Hollinshead
  • KD Validated
View Article
African green monkeyWB

Virol Sin

African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.

Authors - Sheng Chen
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

balawant (Verified Customer) (12-25-2023)

I have used this antibody . This antibody is working great.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western blot and 1:700 for IHC
  • Cell Tissue Type: colon tissue of mice and human
FH

Balawant (Verified Customer) (08-06-2022)

This antibody is working great. I have used this antibody for tissue lysate and cell lysate. it has no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and colon cancer cells
FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 2000
  • Cell Tissue Type: CRC cell line
FH

Iram (Verified Customer) (07-03-2020)

Giving a very sharp and clean result

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human