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  • Western Blot (WB) analysis of various lysates using PVRIG Polyclonal antibody (21448-1-AP)

    WB analysis using 21448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21448-1-AP (PVRIG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PVRIG Polyclonal antibody

PVRIG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21448-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Transmembrane protein PVRIG, Poliovirus receptor-related immunoglobulin domain-containing protein, CD112R, CD112 receptor, C7orf15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inMOLT-4 cells, NK-92 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21448-1-AP targets PVRIG in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PVRIG fusion protein Ag13815 Predict reactive species
Full Name poliovirus receptor related immunoglobulin domain containing
Calculated Molecular Weight 326 aa, 34 kDa
Observed Molecular Weight ~34 kDa
GenBank Accession NumberBC001129
Gene Symbol PVRIG
Gene ID (NCBI) 79037
RRIDAB_2878859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DKI7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PVRIG, also named as CD112R and C7orf15, is an immune suppressive molecule expressed on T and NK cells. It's a cell surface receptor for NECTIN2. PVRIG is a target for the treatment of cancer.

Protocols

Product Specific Protocols
WB protocol for PVRIG antibody 21448-1-APDownload protocol
IHC protocol for PVRIG antibody 21448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols