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Tested Applications
| Positive WB detected in | A549 cells, MCF-7 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17985-1-AP targets PRKX/PRKY in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PRKX/PRKY fusion protein Ag12404 Predict reactive species |
| Full Name | protein kinase, Y-linked |
| Calculated Molecular Weight | 277 aa, 32 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC074852 |
| Gene Symbol | PRKY |
| Gene ID (NCBI) | 5616 |
| RRID | AB_3669286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRKY is a member of the cAMP-dependent serine-threonine protein kinase gene family, which appears to encode a protein related to the catalytic subunit of the cAMP-dependent protein kinases, which are key players in the cellular responses to the second messenger cAMP. A chromosomal aberration involving PRKY is a cause of sex reversal disorder. As a homolog with PRKX, the antibody can recognize endogenous levels of PRKY and PRKX.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRKX/PRKY antibody 17985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
