human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14985-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14985-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14985-1-AP
IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.
IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.
IHC staining of human breast cancer using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 14985-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).
human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14985-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14985-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse liver using 14985-1-AP
IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.
IF/ICC Figures
IF Staining of HeLa using 14985-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PPA1 Polyclonal antibody
Catalog Number
14985-1-AP
Citations
2
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
