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POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15278-1-AP

Synonyms

CHRAC17, CHRAC 17, CHARAC17, AsTP, Arsenic-transactivated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLE3 Polyclonal antibody (15278-1-AP)

    WB analysis using 15278-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15278-1-AP (POLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using POLE3 Polyclonal antibody (15278-1-AP)

    WB analysis of MCF-7 using 15278-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15278-1-AP (POLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using POLE3 Polyclonal antibody (15278-1-AP)

    IP experiment of HepG2 using 15278-1-AP

    IP result of anti-POLE3 (IP:15278-1-AP, 4ug; Detection:15278-1-AP 1:1000) with HepG2 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using POLE3 Polyclonal antibody (15278-1-AP)

    IHC staining of human liver cancer using 15278-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using POLE3 Polyclonal antibody (15278-1-AP)

    IHC staining of human liver cancer using 15278-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLE3 Polyclonal antibody (15278-1-AP)

    IF Staining of HepG2 using 15278-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15278-1-AP (POLE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15278-1-AP targets POLE3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLE3 fusion protein Ag7396
Full Name polymerase (DNA directed), epsilon 3 (p17 subunit)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC003166
Gene Symbol POLE3
Gene ID (NCBI) 54107
RRIDAB_2166751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLE3 antibody 15278-1-APDownload protocol
IHC protocol for POLE3 antibody 15278-1-APDownload protocol
IF protocol for POLE3 antibody 15278-1-APDownload protocol
IP protocol for POLE3 antibody 15278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.

Authors - Chuanyou Yi
View Article
humanWB

Front Oncol

ASF1B Promotes Oncogenesis in Lung Adenocarcinoma and Other Cancer Types.

Authors - Wencheng Zhang
View Article