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PLSCR1 Polyclonal antibody

Name: PLSCR1 Polyclonal antibody
Brand: Proteintech
SKU: 11582-1-AP

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11582-1-AP

Synonyms

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11582-1-AP
WB result of PLSCR1 antibody (11582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLSCR1 transfected HEK-293 cells.
WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11582-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11582-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11582-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11582-1-AP
Mouse lung lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 11582-1-AP
IP result of anti-PLSCR1 (IP:11582-1-AP, 4ug; Detection:11582-1-AP 1:500) with mouse lung tissue lysate 2800ug.
IHC staining of mouse spleen using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11582-1-AP
Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11582-1-AP

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Tested Applications

Positive WB detected in	A549 cells, mouse lung tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, HEK-293 cells, HepG2 cells, human placenta tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	mouse spleen tissue, human kidney tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 12 publications below
IHC	See 4 publications below
IF	See 7 publications below
IP	See 2 publications below

Product Information

11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLSCR1 fusion protein Ag2172
Full Name	phospholipid scramblase 1
Calculated Molecular Weight	318 aa, 35 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC021100
Gene Symbol	PLSCR1
Gene ID (NCBI)	5359
RRID	AB_2165659
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IHC protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IF protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IP protocol for PLSCR1 antibody 11582-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms Authors - Ashley Hernandez-Gutierrez View Article
human	WB	PLoS Pathog Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication. Authors - Weiyu Luo View Article
mouse	WB	J Neurosci Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses Authors - Margherita Caputo KD Validated View Article
human	WB, IF	J Proteome Res An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein. Authors - Yanzhi Yuan KD Validated View Article
human	WB,IF,IP	Cancer Sci Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma. Authors - Wei-Chao Liao KD Validated View Article
mouse	WB	J Leukoc Biol Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation. Authors - Emily Archer Slone View Article

PLSCR1 Polyclonal antibody

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11582-1-AP

WB analysis of HEK-293 using 11582-1-AP

WB analysis using 11582-1-AP

WB analysis using 11582-1-AP

WB analysis of human skeletal muscle using 11582-1-AP

WB analysis of mouse skeletal muscle using 11582-1-AP

WB analysis of HEK-293 using 11582-1-AP

WB analysis using 11582-1-AP

IP experiment of mouse lung using 11582-1-AP

IHC staining of mouse spleen using 11582-1-AP

IHC staining of human pancreas cancer using 11582-1-AP

IHC staining of mouse spleen using 11582-1-AP

IHC staining of human kidney using 11582-1-AP

IHC staining of human kidney using 11582-1-AP

IF Staining of HeLa using 11582-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms

Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.

Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses

An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.

Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation.