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  • KD/KO Validated

PLK2 Polyclonal antibody

PLK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15956-1-AP

Synonyms

PLK 2, PLK2, Polo like kinase 2, Serum inducible kinase, SNK



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, COLO 320 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15956-1-AP targets PLK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK2 fusion protein Ag8710
Full Name polo-like kinase 2 (Drosophila)
Calculated Molecular Weight 685 aa, 78 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC013879
Gene Symbol PLK2
Gene ID (NCBI) 10769
RRIDAB_2237367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

Protocols

Product Specific Protocols
WB protocol for PLK2 antibody 15956-1-APDownload protocol
IP protocol for PLK2 antibody 15956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer.

Authors - Fei Liu
ratWB

J Biochem Mol Toxicol

PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β.

Authors - Yazhi Fan
  • KD Validated
IP

Cell Biol Int

Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells.

Authors - Chun-Hua Wang
mouseWB

J Gastroenterol Hepatol

PLK2 inhibited oxidative stress and ameliorated hepatic ischemia-reperfusion injury through phosphorylating GSK3β

Authors - Wenwen Ge