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- KD/KO Validated
PITX1 Polyclonal antibody
PITX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10873-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
10873-1-AP targets PITX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PITX1 fusion protein Ag1303 |
| Full Name | paired-like homeodomain 1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC009412 |
| Gene Symbol | PITX1 |
| Gene ID (NCBI) | 5307 |
| RRID | AB_10859664 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PITX1, also named as Pituitary homeobox 1 or BFT, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. Bicoid subfamily. PITX1 localizes in the nucleus and may play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PITX1 antibody 10873-1-AP | Download protocol |
| IHC protocol for PITX1 antibody 10873-1-AP | Download protocol |
| IF protocol for PITX1 antibody 10873-1-AP | Download protocol |
| IP protocol for PITX1 antibody 10873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
Hum Mutat Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability. | ||
Endocrinology Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice. | ||
Int J Biochem Cell Biol Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.
| ||
Toxicol Lett All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression. |
