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WB analysis of human testis using 12541-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12541-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 12541-1-AP
IP result of anti-PIP5K1B (IP:12541-1-AP, 4ug; Detection:12541-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

PIP5K1B Polyclonal antibody

Name: PIP5K1B Polyclonal antibody
Brand: Proteintech
SKU: 12541-1-AP

PIP5K1B Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12541-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

PIP5K1 beta, PIP5K1B, PIP5KIbeta, Protein STM 7, PtdIns(4)P 5 kinase 1 beta, STM7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human testis tissue, human brain tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human lung tissue, human heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

12541-1-AP targets PIP5K1B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIP5K1B fusion protein Ag3261 Predict reactive species
Full Name	phosphatidylinositol-4-phosphate 5-kinase, type I, beta
Calculated Molecular Weight	549 aa, 62 kDa
Observed Molecular Weight	62 kDa
GenBank Accession Number	BC030587
Gene Symbol	PIP5K1B
Gene ID (NCBI)	8395
RRID	AB_10734598
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14986
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIP5K1B antibody 12541-1-AP	Download protocol
IHC protocol for PIP5K1B antibody 12541-1-AP	Download protocol
IP protocol for PIP5K1B antibody 12541-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation. Authors - Jaerak Chang View Article
mouse	WB	EMBO J PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling. Authors - Caitlin A Sengelaub View Article
human	WB	EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion. Authors - Takashi Baba KD Validated View Article
human	WB	Cell Chem Biol Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors. Authors - Sindhu Carmen Sivakumaren View Article
		EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion. Authors - Takashi Baba View Article