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PIGK Polyclonal antibody

PIGK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15151-1-AP

Synonyms

Phosphatidylinositol-glycan biosynthesis class K protein, hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using PIGK Polyclonal antibody (15151-1-AP)

    WB analysis of HT-1080 using 15151-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using PIGK Polyclonal antibody (15151-1-AP)

    WB analysis of SMMC-7721 using 15151-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PIGK Polyclonal antibody (15151-1-AP)

    WB analysis of HepG2 using 15151-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PIGK Polyclonal antibody (15151-1-AP)

    IHC staining of human ovary tumor using 15151-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15151-1-AP (PIGK antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PIGK Polyclonal antibody (15151-1-AP)

    IHC staining of human ovary tumor using 15151-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15151-1-AP (PIGK antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHT-1080 cells, SMMC-7721 cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15151-1-AP targets PIGK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGK fusion protein Ag3497
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC026186
Gene Symbol PIGK
Gene ID (NCBI) 10026
RRIDAB_2164682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).

Protocols

Product Specific Protocols
WB protocol for PIGK antibody 15151-1-APDownload protocol
IHC protocol for PIGK antibody 15151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
View Article
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
View Article
monkeyWB

Apoptosis

Golgi-derived extracellular vesicle production induced by SARS-CoV-2 envelope protein

Authors - Qiguang Li
View Article