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PGAP1 Polyclonal antibody

PGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55392-1-AP

Synonyms

Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of human testis using 55392-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of mouse testis using 55392-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of HEK-293 using 55392-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of MCF-7 using 55392-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    WB analysis of mouse testis using 55392-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human testis using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human testis using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human breast cancer using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PGAP1 Polyclonal antibody (55392-1-AP)

    IHC staining of human breast cancer using 55392-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PGAP1 Polyclonal antibody (55392-1-AP)

    IF Staining of HeLa using 55392-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PGAP1 antibody (55392-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, HEK-293 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

55392-1-AP targets PGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name post-GPI attachment to proteins 1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 49-53 kDa, 85-90 kDa
GenBank Accession NumberNM_024989
Gene Symbol PGAP1
Gene ID (NCBI) 80055
RRIDAB_11232412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.

Protocols

Product Specific Protocols
WB protocol for PGAP1 antibody 55392-1-APDownload protocol
IHC protocol for PGAP1 antibody 55392-1-APDownload protocol
IF protocol for PGAP1 antibody 55392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
View Article
humanWB

J Biol Chem

Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors - Takahiro Nakane
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

pitna (Verified Customer) (12-19-2019)

PGAP1According to Uniprot, this protein has 4 isoforms ranging from 49 – 105 KDa. it appears that the regulation of the different isoforms is still an open question in the literatureTo reduce non-specific binding, blots were incubated in Odyssey Blocking buffer (Li-Cor) for 1 hour at room temperature (RT) .

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)
PGAP1 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:55392-1-AP)